验证数据展示
经过测试的应用
Positive IHC detected in | mouse spleen tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse spleen tissue, mouse liver tissue |
推荐稀释比
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84538-1-RR targets F4/80 in IHC, IF-P, ELISA applications and shows reactivity with mouse samples.
Tested Applications | IHC, IF-P, ELISA Application Description |
Tested Reactivity | mouse |
Immunogen | Recombinant protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | EGF-like module containing, mucin-like, hormone receptor-like sequence 1 |
Synonyms | Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1 |
Calculated Molecular Weight | 102 kDa |
GenBank Accession Number | NM_010130.4 |
Gene Symbol | F4/80 |
Gene ID (NCBI) | 13733 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q61549 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.
实验方案
Product Specific Protocols | |
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IHC protocol for F4/80 antibody 84538-1-RR | Download protocol |
IF protocol for F4/80 antibody 84538-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |