F4/80 Recombinant antibody

F4/80 Uni-rAbTM Recombinant Antibody for IHC, IF-P, ELISA
Cat No. 84538-1-RR

产品说明书

CloneNo. 241959B6

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IHC, IF-P, ELISA

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IHC detected inmouse spleen tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue, mouse liver tissue
Planning an IHC experiment? We recommend our IHCeasy Mouse F4/80 Ready-To-Use IHC Kit. Mouse F4/80 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84538-1-RR targets F4/80 in IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 EGF-like module containing, mucin-like, hormone receptor-like sequence 1
别名 Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1
计算分子量102 kDa
GenBank蛋白编号NM_010130.4
基因名称 F4/80
Gene ID (NCBI) 13733
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ61549
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

实验方案

Product Specific Protocols
IHC protocol for F4/80 antibody 84538-1-RRDownload protocol
IF protocol for F4/80 antibody 84538-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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