验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 2 publications below |
产品信息
20963-1-AP targets Factor V in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coagulation factor V (proaccelerin, labile factor) |
| 别名 | F5, PCCF, FVL, Coagulation factor V light chain, Coagulation factor V heavy chain |
| 计算分子量 | 252 kDa |
| 观测分子量 | 145 kDa |
| GenBank蛋白编号 | NM_000130 |
| 基因名称 | F5 |
| Gene ID (NCBI) | 2153 |
| RRID | AB_2878773 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P12259 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
There are 13 blood coagulation factors found in the blood. There are 13 blood coagulation factors found in the blood. F5 has little or no intrinsic procoagulant activity prior to its activation through limited proteolysis by thrombin or F10a at Arg709, Arg1018 and Arg1545. The resulting F5a molecule is a heterodimer that consists of a 105 kDa heavy chain and a 71/74 kDa light chain that are noncovalently associated in a calcium-dependent manner. The intact procofactor molecule (330 kDa) as well as proteins similar in size to the factor Va light chain (74 kDa) and both light chain precursors (280 and 220 kDa) found in factor Va(IIa) were observed. And factor Xa cleaves the factor Va light chain at Arg1765 into fragments of ~30 and ~48 kDa (residues 1546-1765 and 1766-2196, respectively) in the bovine and human systems.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Factor V antibody 20963-1-AP | Download protocol |
| IHC protocol for Factor V antibody 20963-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancers (Basel) The Immunological Contribution of a Novel Metabolism-Related Signature to the Prognosis and Anti-Tumor Immunity in Cervical Cancer. | ||
Thyroid Clinical and Molecular Characterizations of Papillary Thyroid Cancer in Children and Young Adults: a Multicenter Retrospective Study. |
