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F7 Polyclonal antibody

F7 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23058-1-AP

Print datasheet

Synonyms

Factor VII, SPCA, Proconvertin, Eptacog alfa, EC:3.4.21.21

验证数据展示

  • WB analysis using 23058-1-AP

    WB analysis using 23058-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HUVEC using 23058-1-AP

    IF Staining of HUVEC using 23058-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using F7 antibody (23058-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inmouse liver tissue, human placenta tissue, human plasma
Positive IF/ICC detected inHUVEC cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

23058-1-AP targets F7 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen F7 fusion protein Ag19324 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coagulation factor VII (serum prothrombin conversion accelerator)
Synonyms Factor VII, SPCA, Proconvertin, Eptacog alfa, EC:3.4.21.21
Calculated Molecular Weight 466 aa, 52 kDa
Observed Molecular Weight 49-55 kDa
GenBank Accession NumberBC130468
Gene Symbol F7
Gene ID (NCBI) 2155
RRIDAB_2879203
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP08709
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

F7(Coagulation factor VII) is also named as serum prothrombin conversion accelerator(SPCA). It is the key triggering enzyme of the extrinsic coagulation pathway. Hepatocytes are the predominant source of F7 production under normal physiologic conditions, although F7 is also expressed by monocytes/macrophages in inflammation and atherosclerosis(PMID:17018600). This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for F7 antibody 23058-1-APDownload protocol
IF protocol for F7 antibody 23058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.

Authors - Anika Alberts
View Article
mouseWB

Drug Des Devel Ther

Guanxinning Tablet Alleviates Post-Ischemic Stroke Injury Via Regulating Complement and Coagulation Cascades Pathway and Inflammatory Network Mobilization

Authors - Yule Wang
View Article