验证数据展示
经过测试的应用
Positive IF/ICC detected in | human umbilical vein endothelial cells, HUVEC cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : N/A |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
21485-1-AP targets F8 in IF, ELISA applications and shows reactivity with human samples.
经测试应用 | IF, ELISA Application Description |
文献引用应用 | IF |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | F8 fusion protein Ag15772 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | coagulation factor VIII, procoagulant component |
别名 | AHF, Antihemophilic factor, Coagulation factor VIII, DXS1253E, F8, F8B, F8C, FVIII, HEMA, Procoagulant component |
计算分子量 | 2351 aa, 267 kDa |
GenBank蛋白编号 | BC022513 |
基因名称 | F8 |
Gene ID (NCBI) | 2157 |
RRID | AB_2878868 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P00451 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
F8, also named as AHF and F8C, belongs to the multicopper oxidase family. F8, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. F8 can be cleaved to 4 chains: F8a heavy chain, 200 kDa isoform, F8a heavy chain, 92 kDa isoform, F8 B chain and F8a light chain. This antibody can recognize the light chain.
实验方案
Product Specific Protocols | |
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IF protocol for F8 antibody 21485-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |