验证数据展示
经过测试的应用
Positive WB detected in | human plasma tissue, human blood tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:8000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66722-1-Ig targets Factor VIII in WB, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Factor VIII fusion protein Ag15772 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | coagulation factor VIII, procoagulant component |
Synonyms | F8, DXS1253E, Coagulation factor VIII, Antihemophilic factor, AHF |
Calculated Molecular Weight | 2351 aa, 267 kDa |
Observed Molecular Weight | 70 kDa |
GenBank Accession Number | BC022513 |
Gene Symbol | F8 |
Gene ID (NCBI) | 2157 |
RRID | AB_2882073 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P00451 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
F8, also named as AHF and F8C, belongs to the multicopper oxidase family. F8, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. F8 can be cleaved to 4 chains: F8a heavy chain, 200 kDa isoform, F8a heavy chain, 92 kDa isoform, F8 B chain and F8a light chain. This antibody can recognize the light chain.
实验方案
Product Specific Protocols | |
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WB protocol for Factor VIII antibody 66722-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |