验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human brain tissue, human stomach tissue, mouse colon tissue, MKN-45 cells, SGC-7901 cells, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human colon cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy FA2H Ready-To-Use IHC Kit. FA2H primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15452-1-AP targets FA2H in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | FA2H fusion protein Ag7717 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fatty acid 2-hydroxylase |
| 别名 | FAXDC1, Fatty acid hydroxylase domain-containing protein 1, Fatty acid alpha-hydroxylase, Fatty acid 2-hydroxylase, FAAH |
| 计算分子量 | 43 kDa |
| 观测分子量 | 38-43 kDa |
| GenBank蛋白编号 | BC002679 |
| 基因名称 | FA2H |
| Gene ID (NCBI) | 79152 |
| RRID | AB_2101886 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7L5A8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FA2H(Fatty acid 2-hydroxylase) required for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids. The deduced 372-amino acid protein has a calculated molecular mass of 42.8 kD .Both mouse and human FA2H have a C-terminal endoplasmic reticulum (ER) retention motif(PMID: 15658937).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FA2H antibody 15452-1-AP | Download protocol |
| IHC protocol for FA2H antibody 15452-1-AP | Download protocol |
| IF protocol for FA2H antibody 15452-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Cell Quiescent human glioblastoma cancer stem cells drive tumor initiation, expansion, and recurrence following chemotherapy. | ||
Curr Biol FA2H controls cool temperature sensing through modifying membrane sphingolipids in Drosophila | ||
Front Oncol FA2H Exhibits Tumor Suppressive Roles on Breast Cancers via Cancer Stemness Control. | ||
J Med Chem Substrate-based fragment identification for the development of selective, nonpeptidic inhibitors of striatal-enriched protein tyrosine phosphatase. | ||
Biochem Biophys Res Commun Fatty acid 2-hydroxylase (FA2H) as a stimulatory molecule responsible for breast cancer cell migration. |
