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FAAH Polyclonal antibody

FAAH Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 17909-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

Anandamide amidohydrolase 1, FAAH, FAAH 1, FAAH1, fatty acid amide hydrolase, Fatty acid amide hydrolase 1, Oleamide hydrolase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse liver tissue, rat testis tissue, A431 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse testis tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:300
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17909-1-AP targets FAAH in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 FAAH fusion protein Ag12304 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fatty acid amide hydrolase
别名 Anandamide amidohydrolase 1, FAAH, FAAH 1, FAAH1, fatty acid amide hydrolase, Fatty acid amide hydrolase 1, Oleamide hydrolase 1
计算分子量 579 aa, 63 kDa
观测分子量 55-65 kDa
GenBank蛋白编号BC093632
基因名称 FAAH
Gene ID (NCBI) 2166
RRIDAB_2878465
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00519
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.

实验方案

Product Specific Protocols
WB protocol for FAAH antibody 17909-1-APDownload protocol
IHC protocol for FAAH antibody 17909-1-APDownload protocol
IP protocol for FAAH antibody 17909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Elife

p21-activated kinase 1 restricts tonic endocannabinoid signaling in the hippocampus.

Authors - Shuting Xia
humanWB

J Ethnopharmacol

Qihuang Zhuyu Formula Modulates Mitochondrial Function to Inhibit Platelet Activation via Endocannabinoid Signaling Mediated by the SIRT1/PPARα Pathway

Authors - Ruijie Shi
ratWB

J Proteome Res

Joint Synovial Fluid Metabolomics Method to Decipher the Metabolic Mechanisms of Adjuvant Arthritis and Geniposide Intervention.

Authors - Xiang Zhan
mouseWB

Neuroscience

The Endocannabinoid System Contributes to Memory Deficits Induced by Rapid-eye-movement Sleep Deprivation in Adolescent Mice.

Authors - Jinming Zhang
human,mouseWB,IP

Cell Death Differ

FAAH served a key membrane-anchoring and stabilizing role for NLRP3 protein independently of the endocannabinoid system

Authors - Yangyang Zhu
  • KO Validated
humanWB

Cell Death Dis

Combination treatment with FAAH inhibitors/URB597 and ferroptosis inducers significantly decreases the growth and metastasis of renal cell carcinoma cells via the PI3K-AKT signaling pathway

Authors - Junfeng Hao
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