验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat testis tissue, A431 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse testis tissue, human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:300 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17909-1-AP targets FAAH in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | FAAH fusion protein Ag12304 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | fatty acid amide hydrolase |
| Synonyms | Anandamide amidohydrolase 1, FAAH, FAAH 1, FAAH1, fatty acid amide hydrolase, Fatty acid amide hydrolase 1, Oleamide hydrolase 1 |
| Calculated Molecular Weight | 579 aa, 63 kDa |
| Observed Molecular Weight | 55-65 kDa |
| GenBank Accession Number | BC093632 |
| Gene Symbol | FAAH |
| Gene ID (NCBI) | 2166 |
| RRID | AB_2878465 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00519 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAAH antibody 17909-1-AP | Download protocol |
| IHC protocol for FAAH antibody 17909-1-AP | Download protocol |
| IP protocol for FAAH antibody 17909-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife p21-activated kinase 1 restricts tonic endocannabinoid signaling in the hippocampus. | ||
J Ethnopharmacol Qihuang Zhuyu Formula Modulates Mitochondrial Function to Inhibit Platelet Activation via Endocannabinoid Signaling Mediated by the SIRT1/PPARα Pathway | ||
J Proteome Res Joint Synovial Fluid Metabolomics Method to Decipher the Metabolic Mechanisms of Adjuvant Arthritis and Geniposide Intervention. | ||
Neuroscience The Endocannabinoid System Contributes to Memory Deficits Induced by Rapid-eye-movement Sleep Deprivation in Adolescent Mice. | ||
Cell Death Differ FAAH served a key membrane-anchoring and stabilizing role for NLRP3 protein independently of the endocannabinoid system
| ||
Cell Death Dis Combination treatment with FAAH inhibitors/URB597 and ferroptosis inducers significantly decreases the growth and metastasis of renal cell carcinoma cells via the PI3K-AKT signaling pathway |
