验证数据展示
经过测试的应用
Positive WB detected in | human heart tissue, rat heart, mouse heart |
Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse heart tissue |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1900-1:7600 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IF | See 1 publications below |
产品信息
60280-1-Ig targets FABP3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF-P, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | FABP3 fusion protein Ag21483 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor) |
别名 | FABP11, FABP3, Fatty acid binding protein 3, H FABP, M FABP, MDGI, O FABP |
计算分子量 | 15 kDa |
观测分子量 | 15 kDa |
GenBank蛋白编号 | BC007021 |
基因名称 | FABP3 |
Gene ID (NCBI) | 2170 |
RRID | AB_2881398 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P05413 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FABP3 (fatty-acid-binding protein 3), also known as heart-type FABP or mammary-derived growth inhibitor (MDGI), is a small 15-kDa cytoplasmic protein transporting fatty acids and other lipophilic substances from the cytoplasm to the nucleus. It is most ubiquitously expressed in heart and skeletal muscle.
实验方案
Product Specific Protocols | |
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WB protocol for FABP3 antibody 60280-1-Ig | Download protocol |
IHC protocol for FABP3 antibody 60280-1-Ig | Download protocol |
IF protocol for FABP3 antibody 60280-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Cell Mol Med Four transcription profile-based models identify novel prognostic signatures in oesophageal cancer. | ||
Mol Cell Proteomics Quantitative ubiquitylome analysis reveals specificity of RNF111/Arkadia E3 ubiquitin ligase for its degradative substrates SKI and SKIL/SnoN in TGF-β signaling pathway | ||