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验证数据展示

  • WB analysis of MC38 using 12802-1-AP (same clone as 12802-1-PBS)

    WB analysis of MC38 using 12802-1-AP (same clone as 12802-1-PBS)

    WB result of FABP4 antibody (12802-1-AP; 1:800; room temperature for 1.5 hours) with wild-type and FABP4 knockout MC38 cells. This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

  • WB analysis using 12802-1-AP (same clone as 12802-1-PBS)

    WB analysis using 12802-1-AP (same clone as 12802-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12802-1-AP (FABP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brown adipose using 12802-1-AP (same clone as 12802-1-PBS)

    IHC staining of rat brown adipose using 12802-1-AP (same clone as 12802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FABP4/AP2 antibody included
  • IHC staining of rat brown adipose using 12802-1-AP (same clone as 12802-1-PBS)

    IHC staining of rat brown adipose using 12802-1-AP (same clone as 12802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FABP4/AP2 antibody included
  • IHC staining of mouse skin using 12802-1-AP (same clone as 12802-1-PBS)

    IHC staining of mouse skin using 12802-1-AP (same clone as 12802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FABP4/AP2 antibody included
  • IHC staining of mouse skin using 12802-1-AP (same clone as 12802-1-PBS)

    IHC staining of mouse skin using 12802-1-AP (same clone as 12802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12802-1-AP (FABP4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12802-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FABP4/AP2 antibody included
  • Featured Product
  • KD/KO验证

FABP4 Polyclonal antibody, PBS Only

FABP4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12802-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

fatty acid binding protein 4, adipocyte, Fatty acid binding protein 4, ALBP, AFABP, Adipocyte-type fatty acid-binding protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FABP4/AP2 Ready-To-Use IHC Kit. FABP4/AP2 primary antibody included.

产品信息

12802-1-PBS targets FABP4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FABP4 fusion protein Ag3912 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fatty acid binding protein 4, adipocyte
别名 fatty acid binding protein 4, adipocyte, Fatty acid binding protein 4, ALBP, AFABP, Adipocyte-type fatty acid-binding protein
计算分子量 132 aa, 15 kDa
观测分子量 15 kDa
GenBank蛋白编号BC003672
基因名称 FABP4
Gene ID (NCBI) 2167
RRIDAB_2102442
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15090
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.