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FABP4 Polyclonal antibody

FABP4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31129-1-AP

Print datasheet

Synonyms

A FABP, AFABP, ALBP, aP2, FABP4, Fatty acid binding protein 4

验证数据展示

  • WB analysis using 31129-1-AP

    WB analysis using 31129-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31129-1-AP (FABP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy FABP4/AP2 Ready-To-Use IHC Kit. FABP4/AP2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31129-1-AP targets FABP4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fatty acid binding protein 4, adipocyte
Synonyms A FABP, AFABP, ALBP, aP2, FABP4, Fatty acid binding protein 4
Calculated Molecular Weight15 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberNM_001442.3
Gene Symbol FABP4
Gene ID (NCBI) 2167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15090
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.

实验方案

Product Specific Protocols
WB protocol for FABP4 antibody 31129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols