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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis of PC-3 using 68026-1-Ig (same clone as 68026-1-PBS)

    WB analysis of PC-3 using 68026-1-Ig (same clone as 68026-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 68026-1-Ig (same clone as 68026-1-PBS)

    IHC staining of mouse liver using 68026-1-Ig (same clone as 68026-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 68026-1-Ig (same clone as 68026-1-PBS)

    IHC staining of mouse liver using 68026-1-Ig (same clone as 68026-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 68026-1-Ig (same clone as 68026-1-PBS)

    IF Staining of A549 using 68026-1-Ig (same clone as 68026-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FADS2 antibody (68026-1-Ig, Clone: 1G4B10 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

FADS2 Monoclonal antibody, PBS Only

FADS2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68026-1-PBS

产品说明书

CloneNo. 1G4B10

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase, 1G4B10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68026-1-PBS targets FADS2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FADS2 fusion protein Ag27715 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 fatty acid desaturase 2
别名 EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase, 1G4B10
计算分子量 445 aa, 49 kDa
观测分子量42-45 kDa
GenBank蛋白编号BC009011
基因名称 FADS2
Gene ID (NCBI) 9415
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95864
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms produced by alternative splicing.