验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84527-5-RR targets FADS2-Specific in IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | IF/ICC, ELISA Application Description |
经测试反应性 | human |
免疫原 | FADS2-Specific fusion protein Ag27715 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | fatty acid desaturase 2 |
别名 | FADS2, EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase |
计算分子量 | 445 aa, 49 kDa |
GenBank蛋白编号 | BC009011 |
基因名称 | FADS2 |
Gene ID (NCBI) | 9415 |
RRID | AB_3672035 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | O95864 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms and can be detected as 46-52 kDa.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for FADS2-Specific antibody 84527-5-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |