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FAF2 Polyclonal antibody

FAF2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30761-1-AP

Print datasheet

Synonyms

ETEA, FAF2, FAS associated factor 2, KIAA0887, Protein ETEA, UBXD8, UBXN3B

验证数据展示

  • WB analysis using 30761-1-AP

    WB analysis using 30761-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30761-1-AP (FAF2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30761-1-AP targets FAF2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen FAF2 fusion protein Ag33251 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fas associated factor family member 2
Synonyms ETEA, FAF2, FAS associated factor 2, KIAA0887, Protein ETEA, UBXD8, UBXN3B
Calculated Molecular Weight 445 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC014001
Gene Symbol FAF2
Gene ID (NCBI) 23197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CS3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.

实验方案

Product Specific Protocols
WB protocol for FAF2 antibody 30761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols