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  • Featured Product
  • KD/KO Validated

FAK Monoclonal antibody, PBS Only

FAK Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2c

Reactivity

human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2C5B9

Cat No : 66258-1-PBS

Print datasheet

Synonyms

FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2

验证数据展示

  • WB analysis of MCF-7 using 66258-1-Ig (same clone as 66258-1-PBS)

    WB analysis of MCF-7 using 66258-1-Ig (same clone as 66258-1-PBS)

    WB result of FAK antibody (66258-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAK transfected MCF-7 cells. This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

  • WB analysis using 66258-1-Ig (same clone as 66258-1-PBS)

    WB analysis using 66258-1-Ig (same clone as 66258-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66258-1-Ig (FAK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66258-1-Ig (same clone as 66258-1-PBS)

    IHC staining of human breast cancer using 66258-1-Ig (same clone as 66258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66258-1-Ig (FAK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FAK antibody included
  • IHC staining of human breast cancer using 66258-1-Ig (same clone as 66258-1-PBS)

    IHC staining of human breast cancer using 66258-1-Ig (same clone as 66258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66258-1-Ig (FAK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FAK antibody included
  • IF Staining of HUVEC using 66258-1-Ig (same clone as 66258-1-PBS)

    IF Staining of HUVEC using 66258-1-Ig (same clone as 66258-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using FAK antibody (66258-1-Ig, Clone: 2C5B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66258-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FAK Ready-To-Use IHC Kit. FAK primary antibody included.

产品信息

66258-1-PBS targets FAK in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, mouse, rat
Immunogen FAK fusion protein Ag17966 种属同源性预测
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Full Name PTK2 protein tyrosine kinase 2
Synonyms FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2
Calculated Molecular Weight 1052 aa, 119 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC028733
Gene Symbol PTK2
Gene ID (NCBI) 5747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ05397
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.