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FAM103A1 Polyclonal antibody

FAM103A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19422-1-AP

Print datasheet

Synonyms

RNA guanine-7 methyltransferase activating subunit, RAMMET, RAMAC, RAM, HsT19360

验证数据展示

  • WB analysis of A549 using 19422-1-AP

    WB analysis of A549 using 19422-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 19422-1-AP (FAM103A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 19422-1-AP

    IHC staining of mouse lung using 19422-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAMAC antibody included
  • IF Staining of MCF-7 using 19422-1-AP

    IF Staining of MCF-7 using 19422-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 19422-1-AP (FAM103A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse lung using 19422-1-AP

    IHC staining of mouse lung using 19422-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAMAC antibody included
  • IHC staining of human oesophagus cancer using 19422-1-AP

    IHC staining of human oesophagus cancer using 19422-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAMAC antibody included
  • IHC staining of human oesophagus cancer using 19422-1-AP

    IHC staining of human oesophagus cancer using 19422-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAMAC antibody included

经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inmouse lung tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy RAMAC Ready-To-Use IHC Kit. RAMAC primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19422-1-AP targets FAM103A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen FAM103A1 fusion protein Ag13749 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 103, member A1
Synonyms RNA guanine-7 methyltransferase activating subunit, RAMMET, RAMAC, RAM, HsT19360
Calculated Molecular Weight 118 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC003627
Gene Symbol FAM103A1
Gene ID (NCBI) 83640
RRIDAB_2878578
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTL3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM103A1, also named as RNMT-activating mini protein, is a 118 amino acid protein, which belongs to the RAM family. FAM103A1 as a component of mRNA cap binding complex localizes in the nucleus. FAM103A1 is required for efficient mRNA cap methylation and regulates RNMT expression by a post-transcriptional stabilizing mechanism.

实验方案

Product Specific Protocols
WB protocol for FAM103A1 antibody 19422-1-APDownload protocol
IHC protocol for FAM103A1 antibody 19422-1-APDownload protocol
IF protocol for FAM103A1 antibody 19422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Mol Biol

Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E.

Authors - Michael J Osborne
View Article