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FAM105A Polyclonal antibody

FAM105A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25668-1-AP

Print datasheet

Synonyms

FAM105A, NET20, Protein FAM105A

验证数据展示

  • WB analysis of COLO 320 using 25668-1-AP

    WB analysis of COLO 320 using 25668-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25668-1-AP (FAM105A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 25668-1-AP

    IHC staining of human placenta using 25668-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25668-1-AP (FAM105A Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 25668-1-AP

    IHC staining of human placenta using 25668-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25668-1-AP (FAM105A Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inCOLO 320 cells, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25668-1-AP is FAM105A Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen FAM105A fusion protein Ag22243 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 105, member A
Synonyms FAM105A, NET20, Protein FAM105A
Calculated Molecular Weight 356 aa, 42 kDa
Observed Molecular Weight ~35 kDa
GenBank Accession NumberBC011524
Gene Symbol FAM105A
Gene ID (NCBI) 54491
RRIDAB_2880185
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUU6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM105A antibody 25668-1-APDownload protocol
IHC protocol for FAM105A antibody 25668-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols