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OTULIN Polyclonal antibody

OTULIN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27392-1-AP

Print datasheet

Synonyms

FAM105B, OTULIN, Protein FAM105B, Ubiquitin thioesterase otulin

验证数据展示

  • WB analysis using 27392-1-AP

    WB analysis using 27392-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27392-1-AP (OTULIN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27392-1-AP targets OTULIN in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen OTULIN fusion protein Ag22302 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 105, member B
Synonyms FAM105B, OTULIN, Protein FAM105B, Ubiquitin thioesterase otulin
Calculated Molecular Weight 352 aa, 40 kDa
Observed Molecular Weight40-50 kDa
GenBank Accession NumberBC007706
Gene Symbol FAM105B
Gene ID (NCBI) 90268
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BN8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Linear ubiquitination is negatively regulated by OTULIN (OTU deubiquitinase with linear linkage specificity, also known as Fam105b or Gumby), a deubiquitinase specifically catalysing the degradation of linear ubiquitin chains. OUb signalling is antagonised by deubiquitinases (DUBs), which cleave the polyUb signal from substrates to terminate signalling. OTU DUB with linear linkage specificity (OTULIN) and CYLD are the two main DUBs that regulate M1-polyUb signalling. OTULIN exclusively cleaves M1 linkages, whereas CYLD cleaves both M1 and K63 linkages. OTULIN binds directly to the LUBAC subunit HOIP and regulates LUBAC signalling, autoubiquitination, and stability.(PMID: 34625557, PMID: 32231246)

实验方案

Product Specific Protocols
WB protocol for OTULIN antibody 27392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols