• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67926-1-Ig

    WB analysis using 67926-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67926-1-Ig

    IHC staining of mouse brain using 67926-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67926-1-Ig (FAM114A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67926-1-Ig

    IF Staining of HepG2 using 67926-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM114A1 antibody (67926-1-Ig, Clone: 1B11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

FAM114A1 Monoclonal antibody

FAM114A1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67926-1-Ig

产品说明书

CloneNo. 1B11D6
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

Human, rat, mouse and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

FAM114A1, Noxp20, Protein FAM114A1, Protein NOXP20

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inU2OS cells, PC-12 cells, NIH/3T3 cells, A549 cells, HepG2 cells, LNCaP cells, HeLa cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

67926-1-Ig targets FAM114A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IHC
经测试反应性 Human, rat, mouse
文献引用反应性human
免疫原 FAM114A1 fusion protein Ag17289 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 family with sequence similarity 114, member A1
别名 FAM114A1, Noxp20, Protein FAM114A1, Protein NOXP20
计算分子量 563 aa, 61 kDa
观测分子量80 kDa
GenBank蛋白编号BC040452
基因名称 FAM114A1
Gene ID (NCBI) 92689
RRIDAB_2918678
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IWE2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM114A1, also named as Nervous system overexpressed protein 20, is a 563 amino acid protein, which belongs to the FAM114 family. FAM114A1 localizes in the cytoplasm and may play a role in neuronal cell development. The calculated molecular weight of FAM114A1 is 61 kDa, but the modified FAM114A1 may be 70-80 kDa protein.

实验方案

Product Specific Protocols
WB protocol for FAM114A1 antibody 67926-1-IgDownload protocol
IHC protocol for FAM114A1 antibody 67926-1-IgDownload protocol
IF protocol for FAM114A1 antibody 67926-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

RAB42 Promotes Glioma Pathogenesis via the VEGF Signaling Pathway.

Authors - Baoling Liu
View Article