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验证数据展示

  • WB analysis of PC-3 using 83473-3-RR

    WB analysis of PC-3 using 83473-3-RR

    PC-3 cells were subjected to SDS PAGE followed by western blot with 83473-3-RR (FAM116A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 83473-3-RR

    WB analysis of HEK-293 using 83473-3-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 83473-3-RR (FAM116A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83473-3-RR

    FC experiment of HepG2 using 83473-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FAM116A Recombinant antibody (83473-3-RR, Clone:240486B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83473-3-RR

    Affinity and Kinetic Characterization of 83473-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83473-3-RR against Human FAM116A were performed. The affinity constant is 1.43 nM.

FAM116A Recombinant antibody

FAM116A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83473-3-RR

产品说明书

CloneNo. 240486B9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

DENND6A, Protein FAM116A, Protein DENND6A, DENN domain-containing protein 6A, 240486B9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, HEK-293 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83473-3-RR targets FAM116A in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 FAM116A fusion protein Ag22052 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 family with sequence similarity 116, member A
别名 DENND6A, Protein FAM116A, Protein DENND6A, DENN domain-containing protein 6A, 240486B9
计算分子量 608 aa, 70 kDa
观测分子量61 kDa
GenBank蛋白编号BC040291
基因名称 FAM116A
Gene ID (NCBI) 201627
RRIDAB_3671102
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IWF6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM116A has DENN-related domains, which are required for cell migration.

实验方案

Product Specific Protocols
WB protocol for FAM116A antibody 83473-3-RRDownload protocol
FC protocol for FAM116A antibody 83473-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols