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FAM119A Polyclonal antibody

FAM119A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21079-1-AP

Print datasheet

Synonyms

FAM119A, HCA557b, METTL21A, Protein FAM119A

验证数据展示

  • WB analysis using 21079-1-AP

    WB analysis using 21079-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21079-1-AP (FAM119A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 21079-1-AP

    IF Staining of HeLa using 21079-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FAM119A antibody (21079-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 21079-1-AP

    IF Staining of HeLa using 21079-1-AP

    Immunofluorescent analysis of HepG2 cells, using FAM119A antibody 21079-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IHC staining of human liver using 21079-1-AP

    IHC staining of human liver using 21079-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21079-1-AP (FAM119A Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 21079-1-AP

    IHC staining of human liver using 21079-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21079-1-AP (FAM119A Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inK-562 cells, L02 cells, THP-1 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21079-1-AP targets FAM119A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen FAM119A fusion protein Ag15282 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 119, member A
Synonyms FAM119A, HCA557b, METTL21A, Protein FAM119A
Calculated Molecular Weight 218 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC033720
Gene Symbol FAM119A
Gene ID (NCBI) 151194
RRIDAB_10755289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXB1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM119A antibody 21079-1-APDownload protocol
IHC protocol for FAM119A antibody 21079-1-APDownload protocol
IF protocol for FAM119A antibody 21079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

J Biol Chem

Lysine trimethylation regulates 78 kDa glucose-regulated protein proteostasis during endoplasmic reticulum stress.

Authors - Jonas Sieber
View Article
humanWB

J Proteomics

Comparative proteomic analysis of protein methylation provides insight into the resistance of hepatocellular carcinoma to 5-fluorouracil.

Authors - Zhen Liu
View Article