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  • Featured Product
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FAM120A Polyclonal antibody

FAM120A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21529-1-AP

Print datasheet

Synonyms

C9orf10, DNAPTP1, DNAPTP5, FAM120A, KIAA0183, OSSA, Protein FAM120A

验证数据展示

  • WB analysis of siRNA cells using 21529-1-AP

    WB analysis of siRNA cells using 21529-1-AP

    WB result of FAM120A antibody (21529-1-AP) with siRNA control and siRNA-FAM120A.

  • WB analysis of HEK-293 using 21529-1-AP

    WB analysis of HEK-293 using 21529-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21529-1-AP (FAM120A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 21529-1-AP

    IHC staining of human skeletal muscle using 21529-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21529-1-AP (FAM120A antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 21529-1-AP

    IHC staining of human skeletal muscle using 21529-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21529-1-AP (FAM120A antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 21529-1-AP

    IF Staining of HepG2 using 21529-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 21529-1-AP (FAM120A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, siRNA cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21529-1-AP targets FAM120A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen FAM120A fusion protein Ag16149 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 120A
Synonyms C9orf10, DNAPTP1, DNAPTP5, FAM120A, KIAA0183, OSSA, Protein FAM120A
Calculated Molecular Weight 1118 aa, 122 kDa
Observed Molecular Weight 125 kDa
GenBank Accession NumberBC111736
Gene Symbol FAM120A
Gene ID (NCBI) 23196
RRIDAB_10860261
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZB2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM120A antibody 21529-1-APDownload protocol
IHC protocol for FAM120A antibody 21529-1-APDownload protocol
IF protocol for FAM120A antibody 21529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Mol Neurobiol

Monosome Stalls the Translation Process Mediated by IGF2BP in Arcuate Nucleus for Puberty Onset Delay

Authors - Yifen Shen
View Article