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FAM120C Polyclonal antibody

FAM120C Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25987-1-AP

Print datasheet

Synonyms

CXorf17, FAM120C, ORF34, Protein FAM120C, Tumor antigen BJ HCC 21

验证数据展示

  • WB analysis of HepG2 using 25987-1-AP

    WB analysis of HepG2 using 25987-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25987-1-AP (FAM120C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 25987-1-AP

    IP experiment of HepG2 using 25987-1-AP

    IP result of anti-FAM120C (IP:25987-1-AP, 4ug; Detection:25987-1-AP 1:500) with HepG2 cells lysate 3600ug.

  • IF Staining of HepG2 using 25987-1-AP

    IF Staining of HepG2 using 25987-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 25987-1-AP (FAM120C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 25987-1-AP is FAM120C Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen FAM120C fusion protein Ag23235 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 120C
Synonyms CXorf17, FAM120C, ORF34, Protein FAM120C, Tumor antigen BJ HCC 21
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC136413
Gene Symbol FAM120C
Gene ID (NCBI) 54954
RRIDAB_2880321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX05
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM120C antibody 25987-1-APDownload protocol
IF protocol for FAM120C antibody 25987-1-APDownload protocol
IP protocol for FAM120C antibody 25987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Neuron

Inhibition of RNA splicing triggers CHMP7 nuclear entry, impacting TDP-43 function and leading to the onset of ALS cellular phenotypes

Authors - Norah Al-Azzam
View Article