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FAM127B Recombinant antibody

FAM127B Uni-rAbTM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

240716D8

Cat no : 83862-1-RR

Print datasheet

Synonyms

FAM127 A/B/C, FAM127, FAM127C, FAM127A, CXX1

验证数据展示

  • WB analysis using 83862-1-RR

    WB analysis using 83862-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83862-1-RR (FAM127B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 83862-1-RR

    IP experiment of HeLa using 83862-1-RR

    IP result of anti-FAM127B (IP:83862-1-RR, 4ug; Detection:83862-1-RR 1:800) with HeLa cells lysate 1280 ug.

  • Affinity and Kinetic Characterization of 83862-1-RR

    Affinity and Kinetic Characterization of 83862-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83862-1-RR against Human FAM127B were performed. The affinity constant is 13.3 nM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, A549 cells, MCF-7 cells, A2780 cells, U-251 cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83862-1-RR targets FAM127B in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen FAM127B fusion protein Ag13930 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name family with sequence similarity 127, member B
Synonyms FAM127 A/B/C, FAM127, FAM127C, FAM127A, CXX1
Calculated Molecular Weight 113 aa, 13 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC000393
Gene Symbol FAM127B
Gene ID (NCBI) 26071
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BWD3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The FAM127 family currently has three members, namely FAM127A (also called RTL8C and CXX1), FAM127B (also called CXX1B and RTL8A), and FAM127C (also called RTL8B and CXX1C). They are located on chromosomes and their functions are unknown. All three are composed of 113 amino acids and have extremely high sequence similarity. The immunogen of this antibody has a sequence similarity of more than 95% with members of the FAM127 family. Theoretically, the antibody can recognize all members of the FAM127 family.

实验方案

Product Specific Protocols
WB protocol for FAM127B antibody 83862-1-RRDownload protocol
IP protocol for FAM127B antibody 83862-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols