验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, A549 cells, MCF-7 cells, A2780 cells, U-251 cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83862-1-RR targets FAM127B in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | FAM127B fusion protein Ag13930 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 127, member B |
| 别名 | FAM127, FAM127 A/B/C, 240716D8, CXX1, FAM127A |
| 计算分子量 | 113 aa, 13 kDa |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | BC000393 |
| 基因名称 | FAM127B |
| Gene ID (NCBI) | 26071 |
| RRID | AB_3671446 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9BWD3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The FAM127 family currently has three members, namely FAM127A (also called RTL8C and CXX1), FAM127B (also called CXX1B and RTL8A), and FAM127C (also called RTL8B and CXX1C). They are located on chromosomes and their functions are unknown. All three are composed of 113 amino acids and have extremely high sequence similarity. The immunogen of this antibody has a sequence similarity of more than 95% with members of the FAM127 family. Theoretically, the antibody can recognize all members of the FAM127 family.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM127B antibody 83862-1-RR | Download protocol |
| IP protocol for FAM127B antibody 83862-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |