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验证数据展示

  • WB analysis using 28061-1-AP

    WB analysis using 28061-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28061-1-AP (FAM135B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 28061-1-AP

    IHC staining of human kidney using 28061-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28061-1-AP (FAM135B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 28061-1-AP

    IF Staining of mouse brain using 28061-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FAM135B antibody (28061-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

FAM135B Polyclonal antibody

FAM135B Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 28061-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Rat, mouse

应用

WB, IHC, IF-P, ELISA

别名

C8ORFK32, FAM135B, Protein FAM135B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inC6 cells, Neuro-2a cells
Positive IHC detected inhuman kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28061-1-AP targets FAM135B in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Rat, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, Rat, mouse
免疫原 FAM135B fusion protein Ag27897 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 135, member B
别名 C8ORFK32, FAM135B, Protein FAM135B
观测分子量126-155 kDa
GenBank蛋白编号BC106912
基因名称 FAM135B
Gene ID (NCBI) 51059
RRIDAB_2881050
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ49AJ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM135B antibody 28061-1-APDownload protocol
IHC protocol for FAM135B antibody 28061-1-APDownload protocol
IF protocol for FAM135B antibody 28061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols