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验证数据展示

  • WB analysis of K-562 using 24929-1-AP

    WB analysis of K-562 using 24929-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24929-1-AP (FAM155A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 24929-1-AP

    IHC staining of human colon cancer using 24929-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24929-1-AP (FAM155A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 24929-1-AP

    IHC staining of human colon cancer using 24929-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24929-1-AP (FAM155A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAM155A Polyclonal antibody

FAM155A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24929-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

FAM155A, Transmembrane protein FAM155A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24929-1-AP targets FAM155A in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 FAM155A fusion protein Ag21575 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 155, member A
别名 FAM155A, Transmembrane protein FAM155A
计算分子量 458 aa, 51 kDa
观测分子量 49-55 kDa
GenBank蛋白编号BC157830
基因名称 FAM155A
Gene ID (NCBI) 728215
RRIDAB_2879803
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDB1AL88
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM155A is a transmembrane protein with glycosylation. The MW is from 49-55 kDa.

实验方案

Product Specific Protocols
WB protocol for FAM155A antibody 24929-1-APDownload protocol
IHC protocol for FAM155A antibody 24929-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols