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FAM155A Polyclonal antibody

FAM155A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24929-1-AP

Print datasheet

Synonyms

FAM155A, Transmembrane protein FAM155A

验证数据展示

  • WB analysis of K-562 using 24929-1-AP

    WB analysis of K-562 using 24929-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24929-1-AP (FAM155A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 24929-1-AP

    IHC staining of human colon cancer using 24929-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24929-1-AP (FAM155A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 24929-1-AP

    IHC staining of human colon cancer using 24929-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24929-1-AP (FAM155A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24929-1-AP targets FAM155A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen FAM155A fusion protein Ag21575 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 155, member A
Synonyms FAM155A, Transmembrane protein FAM155A
Calculated Molecular Weight 458 aa, 51 kDa
Observed Molecular Weight 49-55 kDa
GenBank Accession NumberBC157830
Gene Symbol FAM155A
Gene ID (NCBI) 728215
RRIDAB_2879803
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDB1AL88
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM155A is a transmembrane protein with glycosylation. The MW is from 49-55 kDa.

实验方案

Product Specific Protocols
WB protocol for FAM155A antibody 24929-1-APDownload protocol
IHC protocol for FAM155A antibody 24929-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols