验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells |
Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
23919-1-AP targets FAM158A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | FAM158A fusion protein Ag20744 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | family with sequence similarity 158, member A |
Synonyms | EMC9, ER membrane protein complex subunit 9, CGI-112, CGI112, CGI 112 |
Calculated Molecular Weight | 208 aa, 23 kDa |
Observed Molecular Weight | 23 kDa |
GenBank Accession Number | BC002491 |
Gene Symbol | FAM158A |
Gene ID (NCBI) | 51016 |
RRID | AB_2879361 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen Affinity purified |
UNIPROT ID | Q9Y3B6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UPF0172 protein FAM158A, also known as c14orf122 or CGI112, is a protein that in humans is encoded by the FAM158A gene located on chromosome 14q11.2. Human FAM158A and its paralogs in other species are part of the uncharacterized protein family UPF0172 family, which is a subset of the JAB1/Mov34/MPN/PAD-1 ubiquitin protease protein family. The MPN superfamily contributes to ubiquitination and de-ubiquitination activity within the cell. The UPF0172 subset no longer has a functional ubiquitination domain and the function is uncharacterized. Fam158a is nearly ubiquitously expressed throughout the human body. The homolog in mice also shows expression throughout the entire body. Several micro-arrays demonstrate the variable expression of Fam158a in response to other factors and in various cancer types. None of this information gives any indication of a specific function but the wide expression of the gene and its high conservation indicate that Fam158a plays an important role in cellular function.
实验方案
Product Specific Protocols | |
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WB protocol for FAM158A antibody 23919-1-AP | Download protocol |
IHC protocol for FAM158A antibody 23919-1-AP | Download protocol |
IF protocol for FAM158A antibody 23919-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |