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验证数据展示

  • WB analysis of human brain using 17147-1-AP

    WB analysis of human brain using 17147-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17147-1-AP (FAM160B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 17147-1-AP

    IHC staining of human brain using 17147-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17147-1-AP (FAM160B2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 17147-1-AP

    IHC staining of human brain using 17147-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17147-1-AP (FAM160B2 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HepG2 using 17147-1-AP

    IP experiment of HepG2 using 17147-1-AP

    IP result of anti-FAM160B2 (IP:17147-1-AP, 4ug; Detection:17147-1-AP 1:300) with HepG2 cells lysate 3200ug.

  • IF Staining of A549 using 17147-1-AP

    IF Staining of A549 using 17147-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 17147-1-AP (FAM160B2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

FAM160B2 Polyclonal antibody

FAM160B2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17147-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Retinoic acid-induced protein 16, RAI16, FP13191, FHIP2B, FHF complex subunit HOOK-interacting protein 2B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17147-1-AP targets FAM160B2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 FAM160B2 fusion protein Ag10920 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 160, member B2
别名 Retinoic acid-induced protein 16, RAI16, FP13191, FHIP2B, FHF complex subunit HOOK-interacting protein 2B
计算分子量 743 aa, 82 kDa
观测分子量 82 kDa
GenBank蛋白编号BC012865
基因名称 FAM160B2
Gene ID (NCBI) 64760
RRIDAB_2246707
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86V87
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM160B2, also named as RAI16, may play a role in early lens development in mammals and in upregulation of genes in CD34+ cells in coronary artery disease patients.

实验方案

Product Specific Protocols
WB protocol for FAM160B2 antibody 17147-1-APDownload protocol
IHC protocol for FAM160B2 antibody 17147-1-APDownload protocol
IF protocol for FAM160B2 antibody 17147-1-APDownload protocol
IP protocol for FAM160B2 antibody 17147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Endocrinol

Identification of retinoid acid induced 16 as a novel androgen receptor target in prostate cancer cells.

Authors - Cui-Ling Ding
View Article