• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 32034-1-AP

    WB analysis using 32034-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32034-1-AP (FAM162A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 32034-1-AP

    IF Staining of A431 using 32034-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FAM162A antibody (32034-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

FAM162A Polyclonal antibody

FAM162A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32034-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Growth and transformation-dependent protein, FWP001, E2-induced gene 5 protein, E2IG5, DC16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inCaki-2 cells, HepG2 cells, RT-4 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32034-1-AP targets FAM162A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 FAM162A fusion protein Ag36569 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 162, member A
别名 Growth and transformation-dependent protein, FWP001, E2-induced gene 5 protein, E2IG5, DC16
计算分子量 154 aa, 17 kDa
观测分子量17 kDa
GenBank蛋白编号BC015060
基因名称 FAM162A
Gene ID (NCBI) 26355
RRIDAB_3670175
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96A26
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Family with sequence similarity 162 member A (FAM162A, also known as E2IG5, HGTD-P, and C3orf28) is a mitochondrial apoptotic protein, which is known as an HIF-1 alpha-responsive pro-apoptotic molecule (PMID: 15082785; 16698020; 22776087). When FAM162A was overexpressed, the hypoxia signal was sent directly to the mitochondria and caused cell death (PMID: 16698020).

实验方案

Product Specific Protocols
WB protocol for FAM162A antibody 32034-1-APDownload protocol
IF protocol for FAM162A antibody 32034-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols