验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, HepG2 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20836-1-AP targets FAM171A2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | FAM171A2 fusion protein Ag14909 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 171, member A2 |
| 别名 | Protein FAM171A2 |
| 计算分子量 | 826 aa, 87 kDa |
| 观测分子量 | 65-75 kDa |
| GenBank蛋白编号 | BC030200 |
| 基因名称 | FAM171A2 |
| Gene ID (NCBI) | 284069 |
| RRID | AB_2878748 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | A8MVW0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FAM171A2 is a key regulator of progranulin expression and modifies the risk of multiple neurodegenerative diseases. In addition, FAM171A2 was considerably expressed in the vascular endothelium and microglia, which are rich in Progranulin (PGRN) (PMID: 33087363).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM171A2 antibody 20836-1-AP | Download protocol |
| IHC protocol for FAM171A2 antibody 20836-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
