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FAM172A Polyclonal antibody

FAM172A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24732-1-AP

Print datasheet

Synonyms

C5orf21, FAM172A, Protein FAM172A

验证数据展示

  • WB analysis using 24732-1-AP

    WB analysis using 24732-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24732-1-AP (FAM172A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 24732-1-AP

    IHC staining of human liver using 24732-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24732-1-AP (FAM172A Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse colon tissue, rat colon tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24732-1-AP is FAM172A Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FAM172A fusion protein Ag20437 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 172, member A
Synonyms C5orf21, FAM172A, Protein FAM172A
Calculated Molecular Weight 416 aa, 48 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC020584
Gene Symbol FAM172A
Gene ID (NCBI) 83989
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WUF8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Family with sequence similarity 172 member A (FAM172A), cloned from human aortic tissues, is subsequently observed in human endothelial and vascular smooth muscle cells, and macrophages. As the imbalance of the cell cycle and apoptosis is closely related to the key characteristics of malignant tumors, FAM172A dysregulation is a potential contributing factor in cell transformation. Recently, FAM172A has been reported to take part in the development of several cancers, such as human liver, colorectal and papillary thyroid cancers. Moreover, FAM172A was upregulated by high glucose in a concentration- and time-dependent manner which indicated that FAM172A may be involved in the pathogenesis of diabetic related diseases. FAM172A has 4 isoforms with the molecular mass of 34-36, 43 and 48 kDa. This antibody was detected at 50-55 kDa on SDS-PAGE. (PMID: 31988090, 26637224)

实验方案

Product Specific Protocols
WB protocol for FAM172A antibody 24732-1-APDownload protocol
IHC protocol for FAM172A antibody 24732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols