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FAM175A Polyclonal antibody

FAM175A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14366-1-AP

Print datasheet

Synonyms

UNQ496/PRO1013, Coiled-coil domain-containing protein 98, CCDC98, BRCA1-A complex subunit Abraxas 1, ABRAXAS1

验证数据展示

  • WB analysis using 14366-1-AP

    WB analysis using 14366-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 14366-1-AP (FAM175A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 14366-1-AP

    IF Staining of MCF-7 using 14366-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 14366-1-AP (FAM175A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293T cells, MCF-7 cells, human testis tissue, Raji cells, Hela cells, Jurkat cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14366-1-AP targets FAM175A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen FAM175A fusion protein Ag5688 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 175, member A
Synonyms UNQ496/PRO1013, Coiled-coil domain-containing protein 98, CCDC98, BRCA1-A complex subunit Abraxas 1, ABRAXAS1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC039573
Gene Symbol FAM175A
Gene ID (NCBI) 84142
RRIDAB_2262501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UWZ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The family with sequence similarity 175 member A gene (FAM175A; also known as ABRAXAS1, CCDC98 and ABRA1), a member of the DNA repair family, contributes to the BRCA1 (BRCA1 DNA repair associated)-dependent DNA damage response and is associated with age at natural menopause.

实验方案

Product Specific Protocols
WB protocol for FAM175A antibody 14366-1-APDownload protocol
IF protocol for FAM175A antibody 14366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

RAP80 phase separation at DNA double-strand break promotes BRCA1 recruitment

Authors - Caolitao Qin
View Article