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验证数据展示

  • WB analysis using 14366-1-AP

    WB analysis using 14366-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 14366-1-AP (FAM175A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 14366-1-AP

    IF Staining of MCF-7 using 14366-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 14366-1-AP (FAM175A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 14366-1-AP

    IF Staining of HeLa using 14366-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FAM175A antibody (14366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FAM175A Polyclonal antibody

FAM175A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14366-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

UNQ496/PRO1013, Coiled-coil domain-containing protein 98, CCDC98, BRCA1-A complex subunit Abraxas 1, ABRAXAS1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, MCF-7 cells, human testis tissue, Raji cells, Hela cells, Jurkat cells
Positive IF/ICC detected inHeLa cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14366-1-AP targets FAM175A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 FAM175A fusion protein Ag5688 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 175, member A
别名 UNQ496/PRO1013, Coiled-coil domain-containing protein 98, CCDC98, BRCA1-A complex subunit Abraxas 1, ABRAXAS1
计算分子量 47 kDa
观测分子量 46 kDa
GenBank蛋白编号BC039573
基因名称 FAM175A
Gene ID (NCBI) 84142
RRIDAB_2262501
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UWZ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The family with sequence similarity 175 member A gene (FAM175A; also known as ABRAXAS1, CCDC98 and ABRA1), a member of the DNA repair family, contributes to the BRCA1 (BRCA1 DNA repair associated)-dependent DNA damage response and is associated with age at natural menopause.

实验方案

Product Specific Protocols
WB protocol for FAM175A antibody 14366-1-APDownload protocol
IF protocol for FAM175A antibody 14366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

RAP80 phase separation at DNA double-strand break promotes BRCA1 recruitment

Authors - Caolitao Qin
View Article