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FAM20A Polyclonal antibody

FAM20A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25258-1-AP

Print datasheet

Synonyms

UNQ9388/PRO34279, Pseudokinase FAM20A, Protein FAM20A, FP2747

验证数据展示

  • IHC staining of human colon cancer using 25258-1-AP

    IHC staining of human colon cancer using 25258-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25258-1-AP (FAM20A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse lung using 25258-1-AP

    WB analysis of mouse lung using 25258-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 25258-1-AP (FAM20A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 25258-1-AP

    IHC staining of human colon cancer using 25258-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25258-1-AP (FAM20A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman colon cancer tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25258-1-AP targets FAM20A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen FAM20A fusion protein Ag19494 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 20, member A
Synonyms UNQ9388/PRO34279, Pseudokinase FAM20A, Protein FAM20A, FP2747
Calculated Molecular Weight 541 aa, 61 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC136686
Gene Symbol FAM20A
Gene ID (NCBI) 54757
RRIDAB_2879991
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MK3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM20A antibody 25258-1-APDownload protocol
IHC protocol for FAM20A antibody 25258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Front Immunol

FAM20A: a potential diagnostic biomarker for lung squamous cell carcinoma

Authors - Yalin Zhang
View Article