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验证数据展示

  • WB analysis using 31694-1-AP

    WB analysis using 31694-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31694-1-AP (FAM210A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 31694-1-AP

    IHC staining of human ovary cancer using 31694-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31694-1-AP (FAM210A antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 31694-1-AP

    IHC staining of human ovary cancer using 31694-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31694-1-AP (FAM210A antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAM210A Polyclonal antibody

FAM210A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31694-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

C18orf19

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse adipose tissue, mouse heart tissue, rat adipose tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31694-1-AP targets FAM210A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FAM210A fusion protein Ag36161 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 18 open reading frame 19
别名 C18orf19
计算分子量30kDa
观测分子量 20 kDa
GenBank蛋白编号NM_001098801
基因名称 C18orf19
Gene ID (NCBI) 125228
RRIDAB_3670081
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96ND0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM210A also known as C18orf19, is a determinant of bone and muscle structure and strength. FAM210A is required for cold-induced mitochondrial cristae remodeling, adaptive thermogenic activity, and brown adipose tissue characteristics(PMID: 37816711). FAM210A is localized to mitochondria and cytoplasm of muscle cells and myotubes(PMID: 29618611).

实验方案

Product Specific Protocols
WB protocol for FAM210A antibody 31694-1-APDownload protocol
IHC protocol for FAM210A antibody 31694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols