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FAM29A Polyclonal antibody

FAM29A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16933-1-AP

Print datasheet

Synonyms

KIAA1574, HAUS6, HAUS augmin-like complex subunit 6, DGT6

验证数据展示

  • WB analysis of Jurkat using 16933-1-AP

    WB analysis of Jurkat using 16933-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16933-1-AP (FAM29A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16933-1-AP

    IF Staining of HeLa using 16933-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FAM29A antibody (16933-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 16933-1-AP

    IF Staining of HeLa using 16933-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FAM29A antibody (16933-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16933-1-AP targets FAM29A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen FAM29A fusion protein Ag10421 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 29, member A
Synonyms KIAA1574, HAUS6, HAUS augmin-like complex subunit 6, DGT6
Calculated Molecular Weight 955 aa, 109 kDa
Observed Molecular Weight 109 kDa
GenBank Accession NumberBC010632
Gene Symbol FAM29A
Gene ID (NCBI) 54801
RRIDAB_1939544
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z4H7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM29A antibody 16933-1-APDownload protocol
IF protocol for FAM29A antibody 16933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biol

Reconstitution and mechanistic dissection of the human microtubule branching machinery.

Authors - Yaqian Zhang
View Article