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FAM3B Polyclonal antibody

FAM3B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27131-1-AP

Print datasheet

Synonyms

C21orf11, C21orf76, Cytokine like protein 2 21, FAM3B, ORF9, Pancreatic derived factor, PANDER, PRED44, Protein FAM3B, UNQ320/PRO365

验证数据展示

  • WB analysis of human milk using 27131-1-AP

    WB analysis of human milk using 27131-1-AP

    human milk were subjected to SDS PAGE followed by western blot with 27131-1-AP (FAM3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 27131-1-AP

    IHC staining of human pancreas using 27131-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 27131-1-AP (FAM3B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas using 27131-1-AP

    IHC staining of human pancreas using 27131-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 27131-1-AP (FAM3B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman milk tissue
Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

27131-1-AP targets FAM3B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen FAM3B fusion protein Ag25955 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 3, member B
Synonyms C21orf11, C21orf76, Cytokine like protein 2 21, FAM3B, ORF9, Pancreatic derived factor, PANDER, PRED44, Protein FAM3B, UNQ320/PRO365
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa, 23 kDa
GenBank Accession NumberBC057829
Gene Symbol FAM3B
Gene ID (NCBI) 54097
RRIDAB_2880766
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP58499
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM3B, also known as Pancreatic-derived factor (PANDER), is a secreted glycoprotein that belongs to the cytokine like-protein family. There are four members in this gene family (FAM3A, FAM3B, FAM3C and FAM3D). As a result of alternative splicing and alternate signal peptide cleavage sites, multiple isoforms are known. FAM3B is selectively expressed at a high level in pancreatic islets and at low levels in the small intestine, prostate, and certain neurons.

实验方案

Product Specific Protocols
WB protocol for FAM3B antibody 27131-1-APDownload protocol
IHC protocol for FAM3B antibody 27131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Int J Gen Med

FAM3B Serves as a Biomarker for the Development and Malignancy of Oral Lichen Planus.

Authors - Wenfang Wang
View Article
humanIHC

Front Pharmacol

A senescence-associated signature refines the classification of different modification patterns and characterization of tumor immune microenvironment infiltration in triple-negative breast cancer

Authors - Renhong Huang
View Article