验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, human brain tissue, human heart tissue, HEK-293 cells, HT-29 cells, HepG2 cells |
| Positive IHC detected in | human pancreas tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 3 publications below |
产品信息
14247-1-AP targets FAM3C in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | FAM3C fusion protein Ag5516 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | family with sequence similarity 3, member C |
| Synonyms | FAM3C, GS3786, ILEI, Interleukin like EMT inducer, Protein FAM3C |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC046932 |
| Gene Symbol | FAM3C |
| Gene ID (NCBI) | 10447 |
| RRID | AB_2278149 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92520 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FAM3C, also known as ILEI, is a secreted protein belonging to the cytokine-like protein family. FAM3C is a signaling cytokine with a GG domain, hydrophobic leader sequence and an N-terminal signal peptide. A change in expression of this protein has been noted in pancreatic cancer-derived cells. FAM3C has been involved in the epithelial to mesenchymal transition, and retinal laminar formation processes in vertebrates. Also, FAM3C may play an important role in inner ear development and thus be involved in cell differentiation and proliferation during inner ear embryogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM3C antibody 14247-1-AP | Download protocol |
| IHC protocol for FAM3C antibody 14247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Proteome Res Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer. | ||
Int J Clin Exp Pathol Overexpression of FAM3C protein as a novel biomarker for epithelial-mesenchymal transition and poor outcome in gastric cancer. | ||
Int J Biol Sci A Novel ER Stress Mediator TMTC3 Promotes Squamous Cell Carcinoma Progression by Activating GRP78/PERK Signaling Pathway | ||
Sci Rep FAM3 family genes are associated with prognostic value of human cancer: a pan-cancer analysis |
