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FAM48A Polyclonal antibody

FAM48A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24419-1-AP

Print datasheet

Synonyms

C13, C13orf19, FAM48A, FP757, p38 interacting protein, P38IP, Protein FAM48A

验证数据展示

  • IHC staining of mouse testis using 24419-1-AP

    IHC staining of mouse testis using 24419-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24419-1-AP (FAM48A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SUPT20H/FAM48A antibody included
  • WB analysis of PC-3 using 24419-1-AP

    WB analysis of PC-3 using 24419-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24419-1-AP (FAM48A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 24419-1-AP

    IHC staining of mouse testis using 24419-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24419-1-AP (FAM48A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SUPT20H/FAM48A antibody included

经过测试的应用

Positive WB detected inPC-3 cells, Jurkat cells
Positive IHC detected inmouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SUPT20H/FAM48A Ready-To-Use IHC Kit. SUPT20H/FAM48A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24419-1-AP targets FAM48A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen FAM48A fusion protein Ag19875 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 48, member A
Synonyms C13, C13orf19, FAM48A, FP757, p38 interacting protein, P38IP, Protein FAM48A
Calculated Molecular Weight 779 aa, 86 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC030686
Gene Symbol FAM48A
Gene ID (NCBI) 55578
RRIDAB_2879537
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NEM7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM48A also named as C13orf19 or SUPT20H is a 779 amino acid protein, which belongs to the SPT20 family. FAM48A is required for MAP kinase p38 activation during gastrulation and for starvation-induced ATG9A trafficking during autophagy. FAM48A localizes in the nucleus and is highly expressed in testis, moderately in brain and pituitary gland.

实验方案

Product Specific Protocols
WB protocol for FAM48A antibody 24419-1-APDownload protocol
IHC protocol for FAM48A antibody 24419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols