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验证数据展示

  • IHC staining of mouse testis using 24419-1-AP

    IHC staining of mouse testis using 24419-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24419-1-AP (FAM48A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SUPT20H/FAM48A antibody included
  • WB analysis of PC-3 using 24419-1-AP

    WB analysis of PC-3 using 24419-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24419-1-AP (FAM48A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 24419-1-AP

    IHC staining of mouse testis using 24419-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24419-1-AP (FAM48A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SUPT20H/FAM48A antibody included

FAM48A Polyclonal antibody

FAM48A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24419-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C13, C13orf19, FAM48A, FP757, p38 interacting protein, P38IP, Protein FAM48A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, Jurkat cells
Positive IHC detected inmouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SUPT20H/FAM48A Ready-To-Use IHC Kit. SUPT20H/FAM48A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24419-1-AP targets FAM48A in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 FAM48A fusion protein Ag19875 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 48, member A
别名 C13, C13orf19, FAM48A, FP757, p38 interacting protein, P38IP, Protein FAM48A
计算分子量 779 aa, 86 kDa
观测分子量 86 kDa
GenBank蛋白编号BC030686
基因名称 FAM48A
Gene ID (NCBI) 55578
RRIDAB_2879537
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NEM7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM48A also named as C13orf19 or SUPT20H is a 779 amino acid protein, which belongs to the SPT20 family. FAM48A is required for MAP kinase p38 activation during gastrulation and for starvation-induced ATG9A trafficking during autophagy. FAM48A localizes in the nucleus and is highly expressed in testis, moderately in brain and pituitary gland.

实验方案

Product Specific Protocols
WB protocol for FAM48A antibody 24419-1-APDownload protocol
IHC protocol for FAM48A antibody 24419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols