• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FAM50A Polyclonal antibody

FAM50A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19849-1-AP

Print datasheet

Synonyms

Protein HXC-26, Protein HXC 26, Protein FAM50A, HXC26, HXC 26

验证数据展示

  • WB analysis using 19849-1-AP

    WB analysis using 19849-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19849-1-AP (FAM50A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat colon using 19849-1-AP

    IHC staining of rat colon using 19849-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 19849-1-AP (FAM50A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FAM50A antibody included
  • IHC staining of rat kidney using 19849-1-AP

    IHC staining of rat kidney using 19849-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19849-1-AP (FAM50A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FAM50A antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inrat colon tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FAM50A Ready-To-Use IHC Kit. FAM50A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19849-1-AP targets FAM50A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FAM50A fusion protein Ag13907 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 50, member A
Synonyms Protein HXC-26, Protein HXC 26, Protein FAM50A, HXC26, HXC 26
Calculated Molecular Weight 325 aa, 39 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC000028
Gene Symbol FAM50A
Gene ID (NCBI) 9130
RRIDAB_10641032
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14320
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM50A belongs to the FAM50 family. It is highly conserved in length and sequence across different species. It is a basic protein containing a nuclear localization signal, and may function as a DNA-binding protein or a transcriptional factor.

实验方案

Product Specific Protocols
WB protocol for FAM50A antibody 19849-1-APDownload protocol
IHC protocol for FAM50A antibody 19849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols