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FAM54A Polyclonal antibody

FAM54A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26569-1-AP

Print datasheet

Synonyms

MTFR2, Mitochondrial fission regulator 2, DUFD1, DUF729 domain-containing protein 1

验证数据展示

  • WB analysis using 26569-1-AP

    WB analysis using 26569-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26569-1-AP (FAM54A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 26569-1-AP

    IF Staining of HeLa using 26569-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FAM54A antibody (26569-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, Kasumi-1 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26569-1-AP targets FAM54A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen FAM54A fusion protein Ag23711 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 54, member A
Synonyms MTFR2, Mitochondrial fission regulator 2, DUFD1, DUF729 domain-containing protein 1
Observed Molecular Weight43 kDa
GenBank Accession NumberBC063688
Gene Symbol FAM54A
Gene ID (NCBI) 113115
RRIDAB_3085885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P444
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Family with sequence similarity 54 member A (FAM54A), also known as MTFR2 or DUFD1, is a mitochondrial outer membrane protein that controls embryonic development and cell differentiation. FAM54A is co-transcribed with core centromere/kinetochore components and plays a critical role in bridging proper mitochondrial fission and chromosome segregation.

实验方案

Product Specific Protocols
WB protocol for FAM54A antibody 26569-1-APDownload protocol
IF protocol for FAM54A antibody 26569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols