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  • Featured Product
  • KD/KO Validated

FAM57B Polyclonal antibody

FAM57B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20760-1-AP

Print datasheet

Synonyms

FAM57B, FP1188, Protein FAM57B

验证数据展示

  • WB analysis using 20760-1-AP

    WB analysis using 20760-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20760-1-AP (FAM57B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 20760-1-AP

    IHC staining of mouse brain using 20760-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20760-1-AP (FAM57B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20760-1-AP

    IHC staining of mouse brain using 20760-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20760-1-AP (FAM57B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse colon tissue, HeLa cells, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20760-1-AP targets FAM57B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen FAM57B fusion protein Ag14707 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 57, member B
Synonyms FAM57B, FP1188, Protein FAM57B
Calculated Molecular Weight 274 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC007892
Gene Symbol FAM57B
Gene ID (NCBI) 83723
RRIDAB_10732598
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ71RH2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM57B antibody 20760-1-APDownload protocol
IHC protocol for FAM57B antibody 20760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

16pdel lipid changes in iPSC-derived neurons and function of FAM57B in lipid metabolism and synaptogenesis

Authors - Danielle L
  • KO Validated
View Article