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  • Featured Product
  • KD/KO Validated

ESYT2 Polyclonal antibody

ESYT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24385-1-AP

Print datasheet

Synonyms

FAM62B, Extended synaptotagmin-2, Extended synaptotagmin 2, E-Syt2, E Syt2

验证数据展示

  • WB analysis using 24385-1-AP

    WB analysis using 24385-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24385-1-AP (FAM62B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 24385-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 24385-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24385-1-AP (ESYT2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FAM62B antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 24385-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 24385-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24385-1-AP (ESYT2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FAM62B antibody included
  • WB analysis of HeLa using 24385-1-AP

    WB analysis of HeLa using 24385-1-AP

    WB result of FAM62B antibody (24385-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM62B transfected HeLa cells.

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, LNCaP cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FAM62B Ready-To-Use IHC Kit. FAM62B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24385-1-AP targets ESYT2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen ESYT2 fusion protein Ag20420 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 62 (C2 domain containing) member B
Synonyms FAM62B, Extended synaptotagmin-2, Extended synaptotagmin 2, E-Syt2, E Syt2
Calculated Molecular Weight 921 aa, 102 kDa
Observed Molecular Weight90-100 kDa
GenBank Accession NumberBC013957
Gene Symbol ESYT2
Gene ID (NCBI) 57488
RRIDAB_2879519
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA0FGR8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ESYT2, also named FAM62B, one of the three-member family of Extended Synaptotagmins that were recently shown to be implicated in the formation of endoplasmic reticulum (ER)-plasma membrane (PM) junctions and in the Ca(2+) dependent regulation of these junctions.

实验方案

Product Specific Protocols
WB protocol for ESYT2 antibody 24385-1-APDownload protocol
IHC protocol for ESYT2 antibody 24385-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

The resealing factor S100A11 interacts with annexins and extended synaptotagmin-1 in the course of plasma membrane wound repair

Authors - Arsila P. K. Ashraf
  • KD Validated
View Article