• Featured Product
  • KD/KO Validated

FAM65B Polyclonal antibody

FAM65B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17015-1-AP

Print datasheet

Synonyms

C6orf32, DIFF40, DIFF48, FAM65B, KIAA0386, PL48, Protein FAM65B, RIPOR2



经过测试的应用

Positive WB detected inhuman skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17015-1-AP targets FAM65B in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen FAM65B fusion protein Ag9991 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 65, member B
Synonyms C6orf32, DIFF40, DIFF48, FAM65B, KIAA0386, PL48, Protein FAM65B, RIPOR2
Calculated Molecular Weight 118 kDa
Observed Molecular Weight 115 kDa, 66 kDa
GenBank Accession NumberBC001232
Gene Symbol FAM65B
Gene ID (NCBI) 9750
RRIDAB_10640810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4F9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM65B is also named as C6orf32, DIFF48, KIAA0386, PL48 and belongs to the FAM65 family.It has 2 isoforms produced by alternative splicing.Isoform 2 play a role in promoting myogenic cell differentiation, cytoskeletal rearrangement and filopodia formation(PMID:17150207). FAM65B is expressed in mononuclear cells, not in myotubes, upon induction of differentiation and cellular fusion, and expression is upregulated during human myoblast differentiation at both the mRNA and protein levels(PMID:17150207).This antibody is specific to the isoform of 119 kDa of FAM65B.

实验方案

Product Specific Protocols
WB protocol for FAM65B antibody 17015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse, ratWB,IF

Proc Natl Acad Sci U S A

FAM65B is a membrane-associated protein of hair cell stereocilia required for hearing.

Authors - Oscar Diaz-Horta
mouseWB

J Cell Sci

Front Signal-Dependent Accumulation of RHOA Inhibitor FAM65B at Leading Edges Polarizes Neutrophils.

Authors - Kun Gao
  • KO Validated
humanWB

FASEB J

Fam65b is important for formation of the HDAC6-dysferlin protein complex during myogenic cell differentiation.

Authors - Anuradha Balasubramanian