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Species Reactivity
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验证数据展示

  • WB analysis using 29811-1-AP

    WB analysis using 29811-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29811-1-AP (FAM73B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MIGA2/FAM73B Polyclonal antibody

MIGA2/FAM73B Polyclonal Antibody for WB, ELISA
Cat No. 29811-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MIGA2, FAM73B, PSEC0112, Protein FAM73B, Mitoguardin 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, U2O2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29811-1-AP targets MIGA2/FAM73B in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen MIGA2/FAM73B fusion protein Ag30577 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 73, member B
Synonyms MIGA2, FAM73B, PSEC0112, Protein FAM73B, Mitoguardin 2
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC009114
Gene Symbol MIGA2/FAM73B
Gene ID (NCBI) 84895
RRIDAB_3086170
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L4E1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MIGA2 (mitoguardin-2) also named as FAM73B, is a mitochondrial protein at contacts with the ER and/or lipid droplets (LDs), and identified as a lipid transporter. MIGA2 localizes to contact sites between mitochondria and the ER or mitochondria and lipid droplets (LDs) (PMID: 36282247). Based on its presence in many tissues, MIGA2 is likely critical for lipid and energy homeostasis in a wide spectrum of cell types (PMID: 31628041).

实验方案

Product Specific Protocols
WB protocol for MIGA2/FAM73B antibody 29811-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols