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FAM76A Polyclonal antibody

FAM76A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20863-1-AP

Print datasheet

Synonyms

FAM76A, Protein FAM76A

验证数据展示

  • WB analysis using 20863-1-AP

    WB analysis using 20863-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20863-1-AP (FAM76A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 20863-1-AP

    IHC staining of mouse testis using 20863-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20863-1-AP (FAM76A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 20863-1-AP

    IHC staining of mouse testis using 20863-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20863-1-AP (FAM76A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, Jurkat cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20863-1-AP targets FAM76A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen FAM76A fusion protein Ag14904 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 76, member A
Synonyms FAM76A, Protein FAM76A
Calculated Molecular Weight 307 aa, 35 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC025768
Gene Symbol FAM76A
Gene ID (NCBI) 199870
RRIDAB_10693681
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TAV0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM76A is a member of FAM76 family. It is reported that FAM76A may be regulated by WTAP. FAM76A is highly expressed in the WTAP high-expression group and also expressed at low levels in the WTAP low-expression group, which can be confirmed by TCGA data (PMID: 32998774).

实验方案

Product Specific Protocols
WB protocol for FAM76A antibody 20863-1-APDownload protocol
IHC protocol for FAM76A antibody 20863-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols