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FAM83G Polyclonal antibody

FAM83G Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29366-1-AP

Print datasheet

Synonyms

Protein FAM83G

验证数据展示

  • WB analysis using 29366-1-AP

    WB analysis using 29366-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29366-1-AP (FAM83G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inBxPC-3 cells, HCT 116 cells, HepG2 cells, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29366-1-AP targets FAM83G in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen FAM83G fusion protein Ag28925 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 83, member G
Synonyms Protein FAM83G
Calculated Molecular Weight 91 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberNM_001039999
Gene Symbol FAM83G
Gene ID (NCBI) 644815
RRIDAB_3669686
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA6ND36
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM83G is a member of the FAM83 protein family, which consists of eight members characterized by sharing a conserved N-terminal domain named DUF1669. This domain is homologous to the phospholipase D catalytic domain but lacks essential catalytic histidine residues, suggesting it is unlikely to have phospholipase activity. FAM83G, in particular, has a consensus motif for serine/threonine protein kinase D1/protein kinase C mu (PKD1/PKCμ) phosphorylation at serine residue 356 (S356), which suggests it may have a unique physiological function among the FAM83 protein family members. Furthermore, FAM83G has been identified as a novel inducer of apoptosis, with its phosphorylation at S356 modulating heat shock protein 27 (HSP27) phosphorylation and apoptosis regulation.

实验方案

Product Specific Protocols
WB protocol for FAM83G antibody 29366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols