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- KD/KO Validated
FAM84B Polyclonal antibody
FAM84B Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MDA-MB-453s cells, Jurkat cells |
| Positive IP detected in | HUVEC cells |
| Positive IHC detected in | human liver cancer tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human oesophagus cancer tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18421-1-AP targets FAM84B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | FAM84B fusion protein Ag13243 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | family with sequence similarity 84, member B |
| Synonyms | FAM84B, NSE2, Protein FAM84B, Protein NSE2 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC052957 |
| Gene Symbol | FAM84B |
| Gene ID (NCBI) | 157638 |
| RRID | AB_2278161 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96KN1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM84B antibody 18421-1-AP | Download protocol |
| IHC protocol for FAM84B antibody 18421-1-AP | Download protocol |
| IP protocol for FAM84B antibody 18421-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Genet Genome-wide profiling of HPV integration in cervical cancer identifies clustered genomic hot spots and a potential microhomology-mediated integration mechanism. | ||
Gigascience Genomic analyses reveal FAM84B and the NOTCH pathway are associated with the progression of esophageal squamous cell carcinoma.
| ||
Proc Natl Acad Sci U S A An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking.
| ||
Aging (Albany NY) FAM84B, amplified in pancreatic ductal adenocarcinoma, promotes tumorigenesis through the Wnt/β-catenin pathway. | ||
