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FAM8A1 Polyclonal antibody

FAM8A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24746-1-AP

Print datasheet

Synonyms

AHCP, FAM8A1, Protein FAM8A1

验证数据展示

  • WB analysis of HEK-293 using 24746-1-AP

    WB analysis of HEK-293 using 24746-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24746-1-AP (FAM8A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 24746-1-AP

    IHC staining of mouse testis using 24746-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24746-1-AP (FAM8A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 24746-1-AP

    IHC staining of mouse testis using 24746-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24746-1-AP (FAM8A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 24746-1-AP is FAM8A1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen FAM8A1 fusion protein Ag20476 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 8, member A1
Synonyms AHCP, FAM8A1, Protein FAM8A1
Calculated Molecular Weight 413 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC047881
Gene Symbol FAM8A1
Gene ID (NCBI) 51439
RRIDAB_2879702
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBU6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM8A1 antibody 24746-1-APDownload protocol
IHC protocol for FAM8A1 antibody 24746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Clin Invest

Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders

Authors - Huilun Helen Wang
View Article
humanWB

Nat Commun

SEL1L-HRD1 interaction is required to form a functional HRD1 ERAD complex

Authors - Liangguang Leo Lin
View Article