验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human cervical cancer tissue, human stomach cancer tissue, mouse intestine, mouse thymus tissue, rat small intestine tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11975-1-AP targets FANCA in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | FANCA fusion protein Ag2598 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Fanconi anemia, complementation group A |
| 别名 | FANCH, FAH, FACA, FAA, FA1 |
| 计算分子量 | 163 kDa |
| 观测分子量 | 163 kDa |
| GenBank蛋白编号 | BC008979 |
| 基因名称 | FANCA |
| Gene ID (NCBI) | 2175 |
| RRID | AB_2101786 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15360 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FANCA, also named as FAA, FACA and FANCH, is a DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FANCA antibody 11975-1-AP | Download protocol |
| IHC protocol for FANCA antibody 11975-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol Fanconi anemia proteins participate in a break-induced-replication-like pathway to counter replication stress.
| ||
Cell Rep C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair | ||
Acta Biochim Biophys Sin (Shanghai) FANCA facilitates G1/S cell cycle advancement, proliferation, migration and invasion in gastric cancer
| ||
Gene Circular RNA Circ_0000119 promotes gastric cancer progression via circ_0000119/miR-502-5p/MTBP axis | ||
Gene Identification of rare heterozygous missense mutations in FANCA in esophageal atresia patients using next-generation sequencing. |
