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Phospho-FANCD2 (Ser330) Polyclonal antibody

Phospho-FANCD2 (Ser330) Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19543-1-AP

Print datasheet

Synonyms

FA D2, FA4, FACD, FAD, FAD2, FANCD, FANCD2, Phospho-FANCD2 (Ser330), Protein FACD2

验证数据展示

  • WB analysis of HL-60 using 19543-1-AP

    WB analysis of HL-60 using 19543-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 19543-1-AP

    IHC staining of human testis using 19543-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 19543-1-AP

    IHC staining of human testis using 19543-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inHL-60 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19543-1-AP targets Phospho-FANCD2 (Ser330) in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fanconi anemia, complementation group D2
Synonyms FA D2, FA4, FACD, FAD, FAD2, FANCD, FANCD2, Phospho-FANCD2 (Ser330), Protein FACD2
Calculated Molecular Weight 166 kDa
Observed Molecular Weight 150-155 kDa
GenBank Accession NumberNM_033084
Gene Symbol FANCD2
Gene ID (NCBI) 2177
RRIDAB_10640576
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXW9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FANCD2, also named as FACD and FACD2, is required for maintenance of chromosomal stability. FANCD2 promotes accurate and efficient pairing of homologs during meiosis. FANCD2 is involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. It may participate in S phase and G2 phase checkpoint activation upon DNA damage. It promotes BRCA2/FANCD1 loading onto damaged chromatin. FANCD2 may also be involved in B-cell immunoglobulin isotype switching. Defects in FANCD2 are a cause of Fanconi anemia (FA) which is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. The antibody is to phospho-Ser(330) of FANCD2.

实验方案

Product Specific Protocols
WB protocol for Phospho-FANCD2 (Ser330) antibody 19543-1-APDownload protocol
IHC protocol for Phospho-FANCD2 (Ser330) antibody 19543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols