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FANCD2 Recombinant antibody

FANCD2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241048G8

Cat no : 83946-4-RR

Print datasheet

Synonyms

FANCD, FAD2, FAD, FACD, FA4

验证数据展示

  • WB analysis using 83946-4-RR

    WB analysis using 83946-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83946-4-RR (FANCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83946-4-RR

    FC experiment of HeLa using 83946-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FANCD2 Recombinant antibody (83946-4-RR, Clone:241048G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83946-4-RR

    Affinity and Kinetic Characterization of 83946-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83946-4-RR against Human FANCD2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, Jurkat cells, U2OS cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83946-4-RR targets FANCD2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen FANCD2 fusion protein Ag29460 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Fanconi anemia, complementation group D2
Synonyms FANCD, FAD2, FAD, FACD, FA4
Calculated Molecular Weight 164 kDa
Observed Molecular Weight150-160 kDa
GenBank Accession NumberNM_001018115
Gene Symbol FANCD2
Gene ID (NCBI) 2177
RRIDAB_3671526
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BXW9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Amongst the FA genes, Fanconi Anemia Group D2 Protein (FANCD2) is the most evolutionarily conserved gene, demonstrating a high sequence similarity from lower eukaryotes to humans. Upon activation, Fanconi anemia group D2 protein (FANCD2) is monoubiquitinated at K561 and works in cooperation with other FA and non-FA proteins to repair DNA damage. In regards to the FA pathway, monoubiquitinated or activated FANCD2 is often portrayed as the functional representative for the functions of activated FA signaling. (PMID: 28825622)

实验方案

Product Specific Protocols
WB protocol for FANCD2 antibody 83946-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols